All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

The Effect of Melatonin on Proliferation of Primary Porcine Cells Expressing Mutated Huntingtin

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F15%3A00476992" target="_blank" >RIV/67985904:_____/15:00476992 - isvavai.cz</a>

  • Alternative codes found

    RIV/67985904:_____/15:00452787

  • Result on the web

    <a href="http://dx.doi.org/10.14735/amcsnn20152S43" target="_blank" >http://dx.doi.org/10.14735/amcsnn20152S43</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.14735/amcsnn20152S43" target="_blank" >10.14735/amcsnn20152S43</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The Effect of Melatonin on Proliferation of Primary Porcine Cells Expressing Mutated Huntingtin

  • Original language description

    According to the recent studies, melatonin might play an important role in Huntington’s disease (HD) and act as a novel therapeutic approach in the treatment of the disease. HD, the inherited neurodegenerative disorder, is accompanied by gradual melatonin reduction as it progresses. Melatonin in normal cells (non tumor) has the anti apoptotic ability due to its antioxidant property and its ability to prevent the activation of p53. Furthermore, melatonin increases the expression of BDNF (brain derived neurotrophic factor) and other neuroprotective factors. The aim of this study was to evaluate the nontoxic dose of melatonin for primary skin fibroblasts isolated from minipigs transgenic for the N terminal part of human mutated huntingtin (TgHD), and the effect of melatonin treatment to these cells exposed to genotoxic stress. Cells were cultured in medium supplemented with different doses of melatonin. Using time lapse microscopy, we estimated the effect of decreasing melatonin concentrations by analyzing the proliferation curves. We show that higher doses of melatonin are toxic for primary porcine fibroblasts. Interestingly, TgHD cells were more sensitive to these doses of melatonin treatment than wild type cells. We evaluated the effective dose of melatonin and demonstrated its rescue proliferative effect on porcine primary cells exposed to genotoxic stress.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Česká a Slovenská neurologie a neurochirurgie

  • ISSN

    1210-7859

  • e-ISSN

  • Volume of the periodical

    78

  • Issue of the periodical within the volume

    Suppl. 2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    6

  • Pages from-to

    43-48

  • UT code for WoS article

  • EID of the result in the Scopus database