The Effect of Melatonin on Proliferation of Primary Porcine Cells Expressing Mutated Huntingtin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F15%3A00476992" target="_blank" >RIV/67985904:_____/15:00476992 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/15:00452787
Result on the web
<a href="http://dx.doi.org/10.14735/amcsnn20152S43" target="_blank" >http://dx.doi.org/10.14735/amcsnn20152S43</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.14735/amcsnn20152S43" target="_blank" >10.14735/amcsnn20152S43</a>
Alternative languages
Result language
angličtina
Original language name
The Effect of Melatonin on Proliferation of Primary Porcine Cells Expressing Mutated Huntingtin
Original language description
According to the recent studies, melatonin might play an important role in Huntington’s disease (HD) and act as a novel therapeutic approach in the treatment of the disease. HD, the inherited neurodegenerative disorder, is accompanied by gradual melatonin reduction as it progresses. Melatonin in normal cells (non tumor) has the anti apoptotic ability due to its antioxidant property and its ability to prevent the activation of p53. Furthermore, melatonin increases the expression of BDNF (brain derived neurotrophic factor) and other neuroprotective factors. The aim of this study was to evaluate the nontoxic dose of melatonin for primary skin fibroblasts isolated from minipigs transgenic for the N terminal part of human mutated huntingtin (TgHD), and the effect of melatonin treatment to these cells exposed to genotoxic stress. Cells were cultured in medium supplemented with different doses of melatonin. Using time lapse microscopy, we estimated the effect of decreasing melatonin concentrations by analyzing the proliferation curves. We show that higher doses of melatonin are toxic for primary porcine fibroblasts. Interestingly, TgHD cells were more sensitive to these doses of melatonin treatment than wild type cells. We evaluated the effective dose of melatonin and demonstrated its rescue proliferative effect on porcine primary cells exposed to genotoxic stress.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Česká a Slovenská neurologie a neurochirurgie
ISSN
1210-7859
e-ISSN
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Volume of the periodical
78
Issue of the periodical within the volume
Suppl. 2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
43-48
UT code for WoS article
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EID of the result in the Scopus database
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