Reclassification of Eubacterium rectale (Hauduroy et al. 1937) Prevot 1938 in a new genus Agathobacter gen. nov as Agathobacter rectalis comb. nov., and description of Agathobacter ruminis sp nov., isolated from the rumen contents of sheep and cows

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F16%3A00458915" target="_blank" >RIV/67985904:_____/16:00458915 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/16:00458915

Result on the web

<a href="http://dx.doi.org/10.1099/ijsem.0.000788" target="_blank" >http://dx.doi.org/10.1099/ijsem.0.000788</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/ijsem.0.000788" target="_blank" >10.1099/ijsem.0.000788</a>

Result language

angličtina

Original language name

Reclassification of Eubacterium rectale (Hauduroy et al. 1937) Prevot 1938 in a new genus Agathobacter gen. nov as Agathobacter rectalis comb. nov., and description of Agathobacter ruminis sp nov., isolated from the rumen contents of sheep and cows

Original language description

Three strains of a butyrate-producing bacterium were isolated from the rumen contents of grazing sheep and cows. The strains were anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming and single flagellate. C-14 : 1, C-14 : (0), C-16 : 0 and C-16 : 1 were the predominant fatty acids. The cell-wall peptidoglycan type was A1 gamma. The DNA G+C content varied from 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between the isolates and Eubacterium rectale, Roseburia hominis and Roseburia intestinalis were found to be 96, 95 and 95 %, respectively. The phylogenetic tree showed that the strains constituted a different taxon, separate from other taxa with validly published names and forming a cluster with strains of Eubacterium rectale. On the basis of phenotypic, chemotaxonomic and phylogenetic results (16S RNA, dnaK, groEL, atpA genes), the isolates are considered to represent a novel species of a new genus of the family Lachnospiraceae, for which the name Agathobacter ruminis gen. nov., sp. nov. is proposed (type strain JK623(T) 5DSM 29029(T) 5LMG 28559(T)). We also propose the transfer of Eubacterium rectale to the new genus as Agathobacter rectalis gen. nov., comb nov. This new genus represents saccharoclastic, chemo-organotrophic and obligatory anaerobic, non-spore-forming rods with Gram-positive membrane. The main fermentation products on peptone yeast glucose (PYG) medium were butyrate, acetate, hydrogen and lactate. The type species of the genus is Agathobacter rectalis gen. nov., comb nov. (Prevot, 1938) with type strain ATCC 33656(T) (=JCM 17463(T)).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Systematic and Evolutionary Microbiology

ISSN

1466-5026

e-ISSN

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Volume of the periodical

66

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

768-773

UT code for WoS article

000372335600036

EID of the result in the Scopus database

2-s2.0-84960464428