Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00479032" target="_blank" >RIV/67985904:_____/17:00479032 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/17:00478269 RIV/00216224:14310/17:00100372 RIV/00216208:11310/17:10369393
Result on the web
<a href="http://dx.doi.org/10.1016/j.ygcen.2017.06.023" target="_blank" >http://dx.doi.org/10.1016/j.ygcen.2017.06.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ygcen.2017.06.023" target="_blank" >10.1016/j.ygcen.2017.06.023</a>
Alternative languages
Result language
angličtina
Original language name
Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)
Original language description
Long term changes in hormonal levels of small and rare animal species can be effectively monitored by non-invasive methods such as immunoenzymatic analysis. These methods generally analyze metabolites instead of the hormone itself and thus do not invade the organism. However, they can be influenced by many factors, so before they are used, they need to be validated. For this purpose we used the ACM challenge test based on stimulating the animal's adrenocortical activity and measuring the consequent increase of the level of its glucocorticoid (stress) hormones. Specifically, we measured concentrations of fecal glucocorticoid metabolites of two house mouse subspecies, Mus musculus musculus and M. m. domesticus. Using polyclonal antibody we investigated the mice's naturally occurring circadian fluctuation and their reaction to the adrenal stimulation. This study confirmed that the selected method is suitable for analysis of fecal corticosterone metabolites in the wild house mouse. More importantly, we revealed a subspecies-specific stress response at the level of corticosterone production: while the significant effect of ACTH was proved in both subspecies, a notable adrenocortical reaction was also elicited by injecting the saline solution in M. m. domesticus. Our results thus highlight the importance of considering potential cryptic variation within the species under study when designing projects on adrenal stress hormone assessments.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
<a href="/en/project/GAP506%2F11%2F1792" target="_blank" >GAP506/11/1792: Population structure, dispersal, and explorative behaviour in the zone of secondary contact of house mice</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
General and Comparative Endocrinology
ISSN
0016-6480
e-ISSN
—
Volume of the periodical
252
Issue of the periodical within the volume
October
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
186-192
UT code for WoS article
000409396400019
EID of the result in the Scopus database
2-s2.0-85024472239