First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes): insights into the karyotype evolution of this ancient fish order
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F18%3A00495749" target="_blank" >RIV/67985904:_____/18:00495749 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/biolinnean/bly098" target="_blank" >http://dx.doi.org/10.1093/biolinnean/bly098</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/biolinnean/bly098" target="_blank" >10.1093/biolinnean/bly098</a>
Alternative languages
Result language
angličtina
Original language name
First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes): insights into the karyotype evolution of this ancient fish order
Original language description
Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELLENCE in Molecular Aspects of the early development of vertebrates</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biological Journal of the Linnean Society
ISSN
0024-4066
e-ISSN
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Volume of the periodical
125
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
83-92
UT code for WoS article
000442937000008
EID of the result in the Scopus database
2-s2.0-85054860856