Behavioral assessment of stress compensation in minipigs transgenic for the huntington gene using cortisol levels: A proof-of-concept study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F18%3A00495756" target="_blank" >RIV/67985904:_____/18:00495756 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3233/JHD-180285" target="_blank" >http://dx.doi.org/10.3233/JHD-180285</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3233/JHD-180285" target="_blank" >10.3233/JHD-180285</a>

Result language

angličtina

Original language name

Behavioral assessment of stress compensation in minipigs transgenic for the huntington gene using cortisol levels: A proof-of-concept study

Original language description

Background: Huntington's disease (HD) is an autosomal-dominant, progressive neurodegenerative disorder with motor, cognitive, behavioral and metabolic symptoms. HD patients exhibit an altered response to stress which is reflected in changes of cortisol levels. Large animal models of HD such as the Libechov minipig are currently explored in preclinical studies to improve translational reliability and assessing behavior is of interest. Objective: This study aimed to investigate whether cortisol metabolism and response to stress are changed in minipigs transgenic for the Huntington gene (tgHD) compared to wildtype (wt) animals suggesting that cortisol may be used as a marker for stress in minipigs. Methods: Thirty-two Libechov minipigs (14 tgHD and 18 wt) were tested before, during and after a stressor, i.e., a hoof trimming procedure, was applied at baseline and after one year. A total of six saliva samples were collected at each assessment and cortisol was measured. In addition, body temperature and respiratory rate were assessed at three pre-determined points during each hoof trimming procedure. Results: All minipigs showed a rise of cortisol in response to the hoof trimming stressor similarly to cortisol changes induced by stress observed in humans. No relevant differences were detected between tgHD and wt minipigs. Conclusion: Cortisol testing for the assessment of stress compensation, e.g., during hoof trimming, is feasible and well tolerated in wt and tgHD minipigs. To elucidate the time profile of cortisol responses to stressors further studies with assessments at multiple time points and exploration of the diurnal profiles of cortisol in minipigs are recommended.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Huntington´s Disease

ISSN

1879-6397

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

151-161

UT code for WoS article

000433980100004

EID of the result in the Scopus database

2-s2.0-85048518927