Colonization of Germ-Free Piglets with Commensal Lactobacillus amylovorus, Lactobacillus mucosae, and Probiotic E. coli Nissle 1917 and Their Interference with Salmonella Typhimurium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00517723" target="_blank" >RIV/67985904:_____/19:00517723 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/19:00517723 RIV/60460709:41210/19:80437

Result on the web

<a href="https://www.mdpi.com/2076-2607/7/8/273" target="_blank" >https://www.mdpi.com/2076-2607/7/8/273</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms7080273" target="_blank" >10.3390/microorganisms7080273</a>

Result language

angličtina

Original language name

Colonization of Germ-Free Piglets with Commensal Lactobacillus amylovorus, Lactobacillus mucosae, and Probiotic E. coli Nissle 1917 and Their Interference with Salmonella Typhimurium

Original language description

Non-typhoid Salmonellae are worldwide spread food-borne pathogens that cause diarrhea in humans and animals. Their multi-drug resistances require alternative ways to combat this enteric pathogen. Mono-colonization of a gnotobiotic piglet gastrointestinal tract with commensal lactobacilli Lactobacillus amylovorus and Lactobacillus mucosae and with probiotic E. coli Nissle 1917 and their interference with S. Typhimurium infection was compared. The impact of bacteria and possible protection against infection with Salmonella were evaluated by clinical signs, bacterial translocation, intestinal histology, mRNA expression of villin, claudin-1, claudin-2, and occludin in the ileum and colon, and local intestinal and systemic levels of inflammatory cytokines IL-8, TNF-alpha, and IL-10. Both lactobacilli colonized the gastrointestinal tract in approximately 100x lower density compare to E. coli Nissle and S. Typhimurium. Neither L. amylovorus nor L. mucosae suppressed the inflammatory reaction caused by the 24 h infection with S. Typhimurium. In contrast, probiotic E. coli Nissle 1917 was able to suppress clinical signs, histopathological changes, the transcriptions of the proteins, and the inductions of the inflammatory cytokines. Future studies are needed to determine whether prebiotic support of the growth of lactobacilli and multistrain lactobacilli inoculum could show higher protective effects.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA13-08803S" target="_blank" >GA13-08803S: Commensal and probiotic bacteria in prevention of enteric infections in early postnatal period</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MICROORGANISMS

ISSN

2076-2607

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

273

UT code for WoS article

000482956300059

EID of the result in the Scopus database

2-s2.0-85074264702