Laser microirradiation as a tool for DNA damage response studies

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00536629" target="_blank" >RIV/67985904:_____/20:00536629 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Laser microirradiation as a tool for DNA damage response studies
Original language description
Double-strand DNA breaks (DSBs) are considered as the most hazardous type of DNA lesions. Recognition of DSBs, temporal cell cycle arrest and activation of repair pathways are crucial steps in response to DNA damage. Errors in any of these steps can lead to adverse consequences affecting genome integrity, e.g. chromosomal aberrations or generation of mutations. The genomic instability caused by severe endpoints of DSBs processing can lead to cell death or development of cancer. Therefore, it is crucial to study all aspects of DNA damage response in cells.nIn the context of studying DNA damage response in cells, DSBs are typically induced either by γ-irradiation or by UV light. DSBs can be also induced by radiomimetic drugs, such as Neocarzinostatin. However, these techniques have several limitations, e.g. inability to induced DSBs only in a specific region of nucleus. Therefore, the laser UV microirradiation technique combined with confocal microscopy was developed to study the DNA damage response in living cells.
Czech name
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Czech description
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Project
<a href="/en/project/LO1609" target="_blank" >LO1609: Models of the Serious Human Diseases: Traumatic Spinal Cord Injury, Huntington’s Disease, Melanoma and Infertility</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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