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Simultaneous targeting of mitochondrial metabolism and immune checkpoints as a new strategy for renal cancer therapy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00556526" target="_blank" >RIV/67985904:_____/22:00556526 - isvavai.cz</a>

  • Alternative codes found

    RIV/86652036:_____/22:00556526 RIV/68378050:_____/22:00556526 RIV/67985823:_____/22:00556526 RIV/00064165:_____/22:10450733 RIV/00216208:11310/22:10450733

  • Result on the web

    <a href="https://onlinelibrary.wiley.com/doi/10.1002/ctm2.645" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/ctm2.645</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/ctm2.645" target="_blank" >10.1002/ctm2.645</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Simultaneous targeting of mitochondrial metabolism and immune checkpoints as a new strategy for renal cancer therapy

  • Original language description

    Mitochondrially targeted tamoxifen (MitoTam) has recently undergone phase 1/1b clinical trial in patients with various solid tumors. Since patients with clear cell renal carcinoma showed the highest response of the tested diagnoses, we studied the effect of MitoTam on renal cancer in vitro and in vivo to reveal its mechanism of action in more detail and to better understand its benefit for patients. Using primarily the murine RenCa renal cancer cell line and the derived syngeneic mouse tumor model, we studied mechanism of MitoTam toxicity including the mode of death, the role of mitochondria in the effects of the agent, and its efficacy in suppressing syngeneic tumors in mice alone and in combination with the immune checkpoint inhibitors (ICIs), monoclonal antibodies blocking PD-1 and PD-L1. Our findings show a complex effect of MitoTam on mitochondrial function and integrity of renal cancer cells. The agent inhibits complex I-dependent respiration and lowers mitochondrial potential, which results in activation of necroptosis as the major mode of cell death. As a consequence, MitoTam reduces growth of renal tumors as well as metastatic spread of tumor cells via specific targeting of malignant tissue in a mouse model. Moreover, combination of MitoTam with immunotherapy to enhance its anti-cancer efficacy shows significantly increased suppression of tumor growth as well as increased survival of experimental animals compared to single agent treatment. Our data provide a mechanistic rationale for testing of both mono and/or combinatorial therapy with MitoTam plus ICIs in renal carcinomas in Phase 2 trial.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30109 - Pathology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Clinical and Translational Medicine

  • ISSN

    2001-1326

  • e-ISSN

    2001-1326

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    8

  • Pages from-to

    e645

  • UT code for WoS article

    000774832800001

  • EID of the result in the Scopus database