Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00558880" target="_blank" >RIV/67985904:_____/22:00558880 - isvavai.cz</a>

Result on the web

<a href="https://jasbsci.biomedcentral.com/articles/10.1186/s40104-022-00723-1" target="_blank" >https://jasbsci.biomedcentral.com/articles/10.1186/s40104-022-00723-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40104-022-00723-1" target="_blank" >10.1186/s40104-022-00723-1</a>

Result language

angličtina

Original language name

Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality

Original language description

Background Ovarian follicular fluids (FFs) contain several kinds of regulatory factors that maintain a suitable microenvironment for oocyte development. Extracellular vesicles (EVs) are among the factors that play essential roles in regulating follicle and oocyte development through their cargo molecules that include microRNAs (miRNAs). This study aimed to investigate small-EV (s-EV) miRNAs in porcine FFs and their potential association with oocyte quality. Methods Individual aspirated oocytes were stained with lissamine green B stain (LB), a vital stain for oocyte quality, and each oocyte was classified as high-quality (unstained, HQ) or low-quality (stained, LQ). FFs corresponding to oocytes were pooled together into HQ and LQ groups. Small-EVs were isolated from FFs, characterized, and their miRNA cargo was identified using the Illumina NovaSeq sequencing platform. Additionally, s-EVs from the HQ and LQ groups were utilized to investigate their effect on oocyte development after co-incubation during in vitro maturation. Results A total of 19 miRNAs (including miR-125b, miR-193a-5p, and miR-320) were significantly upregulated, while 23 (including miR-9, miR-206, and miR-6516) were downregulated in the HQ compared to the LQ group. Apoptosis, p53 signaling, and cAMP signaling were among the top pathways targeted by the elevated miRNAs in the HQ group while oocyte meiosis, gap junction, and TGF-beta signaling were among the top pathways targeted by the elevated miRNAs in the LQ group. The supplementation of small-EVs during maturation does not affect the oocyte developmental rates. However, LQ s-EVs increase the proportion of oocytes with homogeneous mitochondrial distribution and decrease the proportion of heterogeneous distribution. Conclusion Our findings indicated that FF-EVs contain different miRNA cargos associated with oocyte quality and could affect the mitochondrial distribution patterns during oocyte maturation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

<a href="/en/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELLENCE in Molecular Aspects of the early development of vertebrates</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Animal Science and Biotechnology

ISSN

1674-9782

e-ISSN

2049-1891

Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

CN - CHINA

Number of pages

16

Pages from-to

82

UT code for WoS article

000813838300001

EID of the result in the Scopus database

2-s2.0-85132557882