Alteration of primary cilia morphology and associated signalling in ameloblastoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00559671" target="_blank" >RIV/67985904:_____/22:00559671 - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/22:E0109656 RIV/00216224:14310/22:00129706 RIV/61988987:17110/22:A2302ITU RIV/62157124:16170/22:43880046
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S000399692200156X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S000399692200156X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.archoralbio.2022.105499" target="_blank" >10.1016/j.archoralbio.2022.105499</a>
Alternative languages
Result language
angličtina
Original language name
Alteration of primary cilia morphology and associated signalling in ameloblastoma
Original language description
Objectives: Primary cilium is a cellular organelle with growing significance confirmed in tumour biology. Primary cilia have been associated with fine tuning of numerous cell signalling pathways and the role of this structure in cancer initiation and progression is recently at the forefront of attention. Here, we investigated possible alter-ations in the occurrence of primary cilia and changes of associated signalling in ameloblastoma, which represents the most common odontogenic tumour. Methods: We performed immunohistochemistry to assess the number and morphology of primary cilia in ame-loblastoma tissues. The gene expression of key SHH pathway members was analysed by qPCR. As a functional experiment, we treated a primary ameloblastoma cell line by a SHH pathway inhibitor Sonidegib (LDE225). Results: We uncovered differences in primary cilia distribution and appearance in histological subtypes of ameloblastoma with the highest number of ciliated cells in plexiform and follicular subtypes. SHH protein was located close to primary cilia in ameloblastoma epithelial cells and the expression of molecules downstream of SHH signalling was upregulated. Moreover, the inhibition of SHH pathway by Sonidegib caused downregulation of SHH effector gene GLI1 and cell cycle regulator CCND1 in ameloblastoma primary cell line. The inhibition of SHH signalling also altered the expression of molecules involved in intraflagellar transport. Conclusions: In conclusion, our study uncovered alterations in number of ciliated cells and associated signalling in ameloblastoma, which indicate SHH inhibitors as potential therapeutic target to treat this disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30208 - Dentistry, oral surgery and medicine
Result continuities
Project
<a href="/en/project/NU20-08-00205" target="_blank" >NU20-08-00205: Molecular etiopathogenesis of apical periodontitis and odontogenic cystic lesions</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Oral Biology
ISSN
0003-9969
e-ISSN
1879-1506
Volume of the periodical
142
Issue of the periodical within the volume
OCT 2022
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
105499
UT code for WoS article
000831041300002
EID of the result in the Scopus database
2-s2.0-85134517605