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Alteration of primary cilia morphology and associated signalling in ameloblastoma

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00559671" target="_blank" >RIV/67985904:_____/22:00559671 - isvavai.cz</a>

  • Alternative codes found

    RIV/00843989:_____/22:E0109656 RIV/00216224:14310/22:00129706 RIV/61988987:17110/22:A2302ITU RIV/62157124:16170/22:43880046

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S000399692200156X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S000399692200156X?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.archoralbio.2022.105499" target="_blank" >10.1016/j.archoralbio.2022.105499</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Alteration of primary cilia morphology and associated signalling in ameloblastoma

  • Original language description

    Objectives: Primary cilium is a cellular organelle with growing significance confirmed in tumour biology. Primary cilia have been associated with fine tuning of numerous cell signalling pathways and the role of this structure in cancer initiation and progression is recently at the forefront of attention. Here, we investigated possible alter-ations in the occurrence of primary cilia and changes of associated signalling in ameloblastoma, which represents the most common odontogenic tumour. Methods: We performed immunohistochemistry to assess the number and morphology of primary cilia in ame-loblastoma tissues. The gene expression of key SHH pathway members was analysed by qPCR. As a functional experiment, we treated a primary ameloblastoma cell line by a SHH pathway inhibitor Sonidegib (LDE225). Results: We uncovered differences in primary cilia distribution and appearance in histological subtypes of ameloblastoma with the highest number of ciliated cells in plexiform and follicular subtypes. SHH protein was located close to primary cilia in ameloblastoma epithelial cells and the expression of molecules downstream of SHH signalling was upregulated. Moreover, the inhibition of SHH pathway by Sonidegib caused downregulation of SHH effector gene GLI1 and cell cycle regulator CCND1 in ameloblastoma primary cell line. The inhibition of SHH signalling also altered the expression of molecules involved in intraflagellar transport. Conclusions: In conclusion, our study uncovered alterations in number of ciliated cells and associated signalling in ameloblastoma, which indicate SHH inhibitors as potential therapeutic target to treat this disease.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30208 - Dentistry, oral surgery and medicine

Result continuities

  • Project

    <a href="/en/project/NU20-08-00205" target="_blank" >NU20-08-00205: Molecular etiopathogenesis of apical periodontitis and odontogenic cystic lesions</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Archives of Oral Biology

  • ISSN

    0003-9969

  • e-ISSN

    1879-1506

  • Volume of the periodical

    142

  • Issue of the periodical within the volume

    OCT 2022

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    105499

  • UT code for WoS article

    000831041300002

  • EID of the result in the Scopus database

    2-s2.0-85134517605