Commensal Bacteria Impact on Intestinal Toll-like Receptor Signaling in Salmonella-Challenged Gnotobiotic Piglets
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F23%3A00579356" target="_blank" >RIV/67985904:_____/23:00579356 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/23:00579356 RIV/60460709:41210/23:95161
Result on the web
<a href="https://www.mdpi.com/2076-0817/12/11/1293" target="_blank" >https://www.mdpi.com/2076-0817/12/11/1293</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/pathogens12111293" target="_blank" >10.3390/pathogens12111293</a>
Alternative languages
Result language
angličtina
Original language name
Commensal Bacteria Impact on Intestinal Toll-like Receptor Signaling in Salmonella-Challenged Gnotobiotic Piglets
Original language description
Gnotobiotic (GN) animals with simple and defined microbiota can help to elucidate host-pathogen interferences. Hysterectomy-derived germ-free (GF) minipigs were associated at 4 and 24 h post-hysterectomy with porcine commensal mucinolytic Bifidobacterium boum RP36 (RP36) strain or non-mucinolytic strain RP37 (RP37) or at 4 h post-hysterectomy with Lactobacillus amylovorus (LA). One-week-old GN minipigs were infected with Salmonella Typhimurium LT2 strain (LT2). We monitored histological changes in the ileum, mRNA expression of Toll-like receptors (TLRs) 2, 4, and 9 and their related molecules lipopolysaccharide-binding protein (LBP), coreceptors MD-2 and CD14, adaptor proteins MyD88 and TRIF, and receptor for advanced glycation end products (RAGE) in the ileum and colon. LT2 significantly induced expression of TLR2, TLR4, MyD88, LBP, MD-2, and CD14 in the ileum and TLR4, MyD88, TRIF, LBP, and CD14 in the colon. The LT2 infection also significantly increased plasmatic levels of inflammatory markers interleukin (IL)-6 and IL-12/23p40. The previous colonization with RP37 alleviated damage of the ileum caused by the Salmonella infection, and RP37 and LA downregulated plasmatic levels of IL-6. A defined oligo-microbiota composed of bacterial species with selected properties should probably be more effective in downregulating inflammatory response than single bacteria.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA21-15621S" target="_blank" >GA21-15621S: Defined minimal microbiota in protection against foodborne pathogen Salmonella enterica</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Pathogens
ISSN
2076-0817
e-ISSN
2076-0817
Volume of the periodical
12
Issue of the periodical within the volume
11
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
1293
UT code for WoS article
001114559800001
EID of the result in the Scopus database
2-s2.0-85178311819