Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00603154" target="_blank" >RIV/67985904:_____/24:00603154 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00138840 RIV/00669806:_____/24:10488424 RIV/00216208:11140/24:10488424
Result on the web
<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0313514" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0313514</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0313514" target="_blank" >10.1371/journal.pone.0313514</a>

Result language
angličtina
Original language name
Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)
Original language description
Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. Astrocytes maintain neural metabolic support, synapse regulation, blood-brain barrier integrity and immunological homeostasis through intricate interactions with other cells, including neurons, microglia, pericytes and lymphocytes. Due to their extensive intercellular crosstalks, astrocytes are also implicated in the pathogenesis of CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson's disease and Alzheimer's disease. Despite the critical importance of astrocytes in neurodegeneration and neuroinflammation are recognized, the lack of suitable in vitro systems limits their availability for modeling human brain pathologies. Here, we report the time-efficient, reproducible generation of astrocytes from human induced pluripotent stem cells (hiPSCs). Our hiPSC-derived astrocytes expressed characteristic astrocyte markers, such as GFAP, S100b, ALDH1L1 and AQP4. Furthermore, hiPSC-derived astrocytes displayed spontaneous calcium transients and responded to inflammatory stimuli by the secretion of type A1 and type A2 astrocyte-related cytokines.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30103 - Neurosciences (including psychophysiology)

Project
<a href="/en/project/NU20-09-00437" target="_blank" >NU20-09-00437: Identification of changes in glutamatergic pathways specific for sporadic form of Alzheimer's disease in human neurons and astrocytes induced from patient's cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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