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Spaghetti Connections: Synaptonemal Complexes as a Tool to Explore Chromosome Structure, Evolution, and Meiotic Behavior in Fish

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00617041" target="_blank" >RIV/67985904:_____/24:00617041 - isvavai.cz</a>

  • Result on the web

    <a href="https://karger.com/cgr/article/164/1/1/896626/Spaghetti-Connections-Synaptonemal-Complexes-as-a" target="_blank" >https://karger.com/cgr/article/164/1/1/896626/Spaghetti-Connections-Synaptonemal-Complexes-as-a</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1159/000538238" target="_blank" >10.1159/000538238</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Spaghetti Connections: Synaptonemal Complexes as a Tool to Explore Chromosome Structure, Evolution, and Meiotic Behavior in Fish

  • Original language description

    Background: The synaptonemal complex (SC) is a protein axis formed along chromosomes during meiotic prophase to ensure proper pairing and crossing over. SC analysis has been widely used to study the chromosomes of mammals and less frequently of birds, reptiles, and fish. It is a promising method to investigate the evolution of fish genomes and chromosomes as a part of complex approach. Summary: Compared with conventional metaphase chromosomes, pachytene chromosomes are less condensed and exhibit pairing between homologous chromosomes. These features of SCs facilitate the study of the small chromosomes that are typical in fish. Moreover, it allows the study of heteromorphisms in sex chromosomes and supernumerary chromosomes. In addition, it enables the investigation of the pairing between orthologous chromosomes in hybrids, which is crucial for uncovering the causes of hybrid sterility and asexual reproduction, such as gynogenesis or hybridogenesis. However, the application of SC analysis to fish chromosomes is limited by the associated complications. First, in most fish, meiosis does not occur during every season and life stage. Second, different SC preparation methods are optimal for different fish species. Third, commercial antibodies targeting meiotic proteins have been primarily developed against mammalian antigens, and not all of them are suitable for fish chromosomes. Key Messages: In the present review, we provide an overview of the methods for preparing fish SCs and highlight important studies using SC analysis in fish. This study will be valuable for planning and designing research that applies SC analysis to fish cytogenetics and genomics.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10613 - Zoology

Result continuities

  • Project

    <a href="/en/project/GF23-07028K" target="_blank" >GF23-07028K: Breaking the rules: modification of sexual reproduction pathways in vertebrates.</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2024

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Cytogenetic and Genome Research

  • ISSN

    1424-8581

  • e-ISSN

    1424-859X

  • Volume of the periodical

    164

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    15

  • Pages from-to

    1-15

  • UT code for WoS article

    001241967800001

  • EID of the result in the Scopus database

    2-s2.0-85194442894