Cross-amplification and multiplexing of SSR markers for Alnus glutinosa and A. incana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F14%3A00432241" target="_blank" >RIV/67985939:_____/14:00432241 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41330/14:64172

Result on the web

<a href="http://dx.doi.org/10.1007/s11295-014-0727-z" target="_blank" >http://dx.doi.org/10.1007/s11295-014-0727-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11295-014-0727-z" target="_blank" >10.1007/s11295-014-0727-z</a>

Result language

angličtina

Original language name

Cross-amplification and multiplexing of SSR markers for Alnus glutinosa and A. incana

Original language description

We investigated 39 previously developed Betula, Alnus, and Corylus simple sequence repeat (SSR) markers for their utility in the cross-generic amplification of two European alder species, i.e., Alnus glutinosa and A. incana. Of these markers, ten loci had successful amplification within Alnus species. Finally, we designed two multiplexes composed of eight and nine loci for A. glutinosa and A. incana, respectively. Multiplexes were tested on 100 samples from five different populations of each species across Europe. The majority of loci had a relatively high genetic diversity, were in Hardy-Weinberg equilibrium, and showed low error rates and low occurrence of null alleles. By comparing sequences of source species and both Alnus species, we concluded that repeat motifs of five of these ten loci differed from those described for the source species. These differences represent mainly the modifications of the original motifs and affected compound or interrupted repeats as well as pure ones.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

<a href="/en/project/GAP504%2F11%2F0402" target="_blank" >GAP504/11/0402: Postglacial colonization of Alnus glutinosa and Alnus incana: analysis of DNA variation and fossil records</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Tree Genetics & Genomes

ISSN

1614-2942

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

865-873

UT code for WoS article

000339906200008

EID of the result in the Scopus database

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