Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F19%3A00510132" target="_blank" >RIV/67985939:_____/19:00510132 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10396400
Result on the web
<a href="http://hdl.handle.net/11104/0302060" target="_blank" >http://hdl.handle.net/11104/0302060</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3897/mycokeys.55.35682" target="_blank" >10.3897/mycokeys.55.35682</a>
Alternative languages
Result language
angličtina
Original language name
Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica
Original language description
Seagrasses provide invaluable ecosystem services yet very little is known about their root mycobiont diversity and distribution. Here we focused on the dominant Mediterranean seagrass Posidonia oceanica and assessed its root mycobiome at 32 localities covering most of the ecoregions in the NW Mediterranean Sea using light and scanning electron microscopy and tag-encoded 454-pyrosequencing. Microscopy revealed that the recently discovered dark septate endophytic association specific for P. oceanica is present at all localities and pyrosequencing confirmed that the P. oceanica root mycobiome is dominated by a single undescribed pleosporalean fungus, hitherto unknown from other hosts and ecosystems. Its numerous slow-growing isolates were obtained from surface-sterilised root segments at one locality and after prolonged cultivation, several of them produced viable sterile mycelium. To infer their phylogenetic relationships we sequenced and analysed the large (LSU) and small (SSU) subunit nrDNA, the ITS nrDNA and the DNA-directed RNA polymerase II (RPB2). The fungus represents an independent marine biotrophic lineage in the Aigialaceae (Pleosporales) and is introduced here as Posidoniomyces atricolor gen. et sp. nov. Its closest relatives are typically plantassociated saprobes from marine, terrestrial and freshwater habitats in Southeast Asia and Central America. This study expands our knowledge and diversity of the Aigialaceae, adds a new symbiotic lifestyle to this family and provides a formal name for the dominant root mycobiont of the dominant Mediterranean seagrass.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10617 - Marine biology, freshwater biology, limnology
Result continuities
Project
<a href="/en/project/LO1417" target="_blank" >LO1417: Centre of Experimental Plant Biology of CU</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MycoKeys
ISSN
1314-4057
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
Jun 26
Country of publishing house
BG - BULGARIA
Number of pages
28
Pages from-to
59-86
UT code for WoS article
000473091600001
EID of the result in the Scopus database
2-s2.0-85069044167