Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00532233" target="_blank" >RIV/67985939:_____/20:00532233 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0310806" target="_blank" >http://hdl.handle.net/11104/0310806</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2020.05.135" target="_blank" >10.1016/j.ijbiomac.2020.05.135</a>

Result language

angličtina

Original language name

Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp

Original language description

Complex structure of cyanobacterium Nostoc sp. exopolysaccharide (EPS), with apparent molecular weight 214 × 103 g/mol, can be deduced from its composition. Chemical and NMR analyses found four dominant sugarmonomers, namely (1→ 4)-linked α-L-arabinopyranose, β-D-glucopyranose, β-D-xylopyranose and (1→ 3)-linked β-D-mannopyranose, two different uronic acids and a lactyl group, with (1→4,6)-linked β-D-glucopyranose as the only branch point suggest a complex structure of this polymer. The dominant uronic acid is α-linked, but it remained unidentified. β-D-Glucuronic acid was present in lower amount. Their position as well as that of lactyl remained undetermined too. Different doses of orally administered EPS in guinea pigs evoked a significant decrease in cough effort and a decrease in airway reactivity. The antitussive efficacy and bronchodilator effect of higher doses of EPS were found to be similar to that of the antitussive drug codeine and the antiasthmatic salbutamol. Without significant cytotoxicity on the RAW264.7 cells, EPS stimulated themacrophage cells to produce pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and prostaglandins (PGs) and nitric oxide (NO) via induction of COX-2 and iNOS expression, respectively, suggesting that this biopolymer potentiates an early innate immune response and can therefore be used as a new immune modulator.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/TE01020080" target="_blank" >TE01020080: Biorefinery Research Centre of Competence</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

—

Volume of the periodical

160

Issue of the periodical within the volume

OCT 1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

364-371

UT code for WoS article

000564748800003

EID of the result in the Scopus database

2-s2.0-85085972436