Molecular quantification of arbuscular mycorrhizal fungal root colonization

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00538085" target="_blank" >RIV/67985939:_____/20:00538085 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/978-1-0716-0603-2" target="_blank" >https://doi.org/10.1007/978-1-0716-0603-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0603-2" target="_blank" >10.1007/978-1-0716-0603-2</a>

Result language
angličtina
Original language name
Molecular quantification of arbuscular mycorrhizal fungal root colonization
Original language description
Specific quantification of root-colonizing arbusular mycorrhizal fungi (AMF) by quantitative real-time PCR is a high-throughput technique, most suitable for determining abundances of AMF species or isolates in previously characterized experimental systems. The use of a suitable assay is crucial for a correct data collection but also highly specific for each experimental system and is therefore covered by general recommendations. Subsequently, specific steps are described for the validation of the assay using a standard dilution series, the determination of appropriate dilution sof DNA extrracts from roots, and the quantification of the gene copy numbers in samples including calculations.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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