Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F23%3A00571563" target="_blank" >RIV/67985939:_____/23:00571563 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/23:10468043

Result on the web

<a href="https://doi.org/10.1186/s40529-023-00374-z" target="_blank" >https://doi.org/10.1186/s40529-023-00374-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40529-023-00374-z" target="_blank" >10.1186/s40529-023-00374-z</a>

Result language

angličtina

Original language name

Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds

Original language description

Vanilla planifolia is the most widely cultivated species of vanilla with high economic importance. However, seed germination under artificial conditions is difficult and yields low germination percentages. The seeds are adapted to endozoochorous dispersal, and we therefore tried to simulate the conditions in the digestive tract by acid scarification of seeds.Immature seeds lacking dormancy, used as a control, showed the highest germination percentage. Among the treatments tested for mature seeds, the hydrochloric acid treatments were significantly the best in breaking dormancy and inducing germination, irrespective of the acid concentration and the presence of pepsin. Conventional treatment with a hypochlorite solution induced much lower germination percentage. Sulphuric acid at concentration 50% was too strong and caused damage to the seeds. Important factor is also high cultivation temperature 30 degrees C as there was nearly no germination at 25 degrees C.Our protocol significantly improves the efficiency of generative propagation of vanilla and allows for significantly higher germination percentages than previously described. The strongly positive effect of hydrochloric acid may be related to the adaptation of seeds to endozoochorous dispersal.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Botanical Studies

ISSN

1999-3110

e-ISSN

1999-3110

Volume of the periodical

64

Issue of the periodical within the volume

1

Country of publishing house

TW - TAIWAN (PROVINCE OF CHINA)

Number of pages

10

Pages from-to

9

UT code for WoS article

000969413200001

EID of the result in the Scopus database

2-s2.0-85153318026