NMR analysis of duplex d(CGCGATCGCG)2 modified by lambda- and delta-[Ru(bpy)2(m-GHK)]Cl2 and DNA photocleavage study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F07%3A00081006" target="_blank" >RIV/68081707:_____/07:00081006 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
NMR analysis of duplex d(CGCGATCGCG)2 modified by lambda- and delta-[Ru(bpy)2(m-GHK)]Cl2 and DNA photocleavage study
Original language description
The interaction of the diastereomeric complexes delta-[Ru(bpy)2(m-GHK)]Cl2 and lambda-[Ru(bpy)2(m-GHK)]Cl2 (bpy is 2,2'-bipyridine, GHK is glycine-1-histidine-1-lysine) with the deoxyribonucleotide duplex d(5'-CGCGATCGCG)2 was characterized by means of 1H NMR spectroscopy. In addition, photocleavage studies were also conducted with the plasmid pUC19 and a 158-bp restriction fragment, showing that both diastereomers cleave DNA with similar efficiency, attacking mainly the guanines of the sequence probably by generating active oxygen species.
Czech name
Analýza pomocí NMR duplexu d(CGCGATCGCG)2 modifikovaného komplexy lambda- a delta-[Ru(bpy)2)m-GHK)]Cl2 a studie fotolýzy DNA
Czech description
Interakce diastereomerních komplexů delta-[Ru(bpy)2(m-GHK)]Cl2 a lambda-[Ru(bpy)2(m-GHK)]Cl2 (bpy je 2,2'-bipyridine, GHK je glycine-1-histidine-1-lysine) s deoxyribonukleotidovým duplexem d(5'-CGCGATCGCG)2 byla charakterizována pomocí 1H NMR spectroscopie. Kromě toho byly provedeny fotolytické studie s plasmidovou DNA pUC19 a restrikčním fragmentem dlouhým 158 párů bází, které ukázaly, že oba diastereomery štěpí DNA s podobnou účinností zejména v místech guaninových zbytků pravděpodobně za vzniku aktivních forem kyslíku.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Inorganic Chemistry
ISSN
0949-8257
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
14
Pages from-to
279-292
UT code for WoS article
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EID of the result in the Scopus database
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