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NMR analysis of duplex d(CGCGATCGCG)2 modified by lambda- and delta-[Ru(bpy)2(m-GHK)]Cl2 and DNA photocleavage study

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F07%3A00081006" target="_blank" >RIV/68081707:_____/07:00081006 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    NMR analysis of duplex d(CGCGATCGCG)2 modified by lambda- and delta-[Ru(bpy)2(m-GHK)]Cl2 and DNA photocleavage study

  • Original language description

    The interaction of the diastereomeric complexes delta-[Ru(bpy)2(m-GHK)]Cl2 and lambda-[Ru(bpy)2(m-GHK)]Cl2 (bpy is 2,2'-bipyridine, GHK is glycine-1-histidine-1-lysine) with the deoxyribonucleotide duplex d(5'-CGCGATCGCG)2 was characterized by means of 1H NMR spectroscopy. In addition, photocleavage studies were also conducted with the plasmid pUC19 and a 158-bp restriction fragment, showing that both diastereomers cleave DNA with similar efficiency, attacking mainly the guanines of the sequence probably by generating active oxygen species.

  • Czech name

    Analýza pomocí NMR duplexu d(CGCGATCGCG)2 modifikovaného komplexy lambda- a delta-[Ru(bpy)2)m-GHK)]Cl2 a studie fotolýzy DNA

  • Czech description

    Interakce diastereomerních komplexů delta-[Ru(bpy)2(m-GHK)]Cl2 a lambda-[Ru(bpy)2(m-GHK)]Cl2 (bpy je 2,2'-bipyridine, GHK je glycine-1-histidine-1-lysine) s deoxyribonukleotidovým duplexem d(5'-CGCGATCGCG)2 byla charakterizována pomocí 1H NMR spectroscopie. Kromě toho byly provedeny fotolytické studie s plasmidovou DNA pUC19 a restrikčním fragmentem dlouhým 158 párů bází, které ukázaly, že oba diastereomery štěpí DNA s podobnou účinností zejména v místech guaninových zbytků pravděpodobně za vzniku aktivních forem kyslíku.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biological Inorganic Chemistry

  • ISSN

    0949-8257

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    14

  • Pages from-to

    279-292

  • UT code for WoS article

  • EID of the result in the Scopus database