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Beta-Arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F07%3A00082920" target="_blank" >RIV/68081707:_____/07:00082920 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/07:00025662

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Beta-Arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo

  • Original language description

    The phosphoprotein dishevelled (Dvl) is an integral part of Wnt signaling and has recently been shown to interact with the multifunctional scaffolding protein beta-arrestin. MEFs lacking beta-arrestins were able to phosphorylate Lrp6 in response to Wnt-3a, but decreased the activation of Dvl and blocked beta-catenin signaling. Furthermore, treatment of xenopus laevis embryos with beta-arrestin morpholinos reduced the activation of endogenous beta-catenin, decreased the expression of the beta-catenin target gene, xnr3, and blocked axis duplication induced by X-Wnt-8, and other molecules. Thus, our results identify beta-arrestin as a necessary component for Wnt/beta-catenin signaling.

  • Czech name

    Beta-arrestin je nezbytnou složkou Wnt/beta-kateninového signálování jak in vitro, tak in vivo

  • Czech description

    Fosfoprotein dishevelled (Dvl), který je integrální součástí Wnt dráhy fyzicky interaguje s multifunkčním proteinem beta-arrestinem. MEF buňky, které nemají beta-arrestiny, jsou sice schopné po přidání Wnt-3a fosforylace proteinu Lrp6 v membráně, ale vykazují sníženou aktivaci Dvl a žádnou aktivaci beta-cateninu. Navíc deplece beta-arrestinu v embryích žab Xenopus laevis snižuje aktivaci endogenního beta-cateninu, expresi Xnr3 a blokuje zdvojení tělní osy, která je indukována X-Wnt8 i jinými aktivátorybeta-catenin dráhy. Naše výsledky tak identifikují beta-arrestin jako nezbytnou součást Wnt/beta-catenin dráhy in vitro a in vivo.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Proceedings of the National Academy of Sciences of the United States of America

  • ISSN

    0027-8424

  • e-ISSN

  • Volume of the periodical

    104

  • Issue of the periodical within the volume

    16

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

    6690-6695

  • UT code for WoS article

  • EID of the result in the Scopus database