Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F13%3A00422432" target="_blank" >RIV/68081707:_____/13:00422432 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.4161/epi.24613" target="_blank" >http://dx.doi.org/10.4161/epi.24613</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4161/epi.24613" target="_blank" >10.4161/epi.24613</a>
Alternative languages
Result language
angličtina
Original language name
Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture
Original language description
In plants, silencing is usually accompanied by DNA methylation and heterochromatic histone marks. We studied these epigenetic modifications in different epialleles of 35S promoter (P35S)-driven tobacco transgenes. In locus 1, the T-DNA was organized as an inverted repeat, and the residing neomycin phosphotransferase II reporter gene (P35S-nptII) was silenced at the posttranscriptional (PTGS) level. Transcriptionally silenced (TGS) epialleles were generated by trans-acting RNA signals in hybrids or in acallus culture. PTGS to TGS conversion in callus culture was accompanied by loss of the euchromatic H3K4me3 mark in the transcribed region of locus 1, but this change was not transmitted to the regenerated plants from these calli. In contrast, cytosine methylation that spread from the transcribed region into the promoter was maintained in regenerants. Also, the TGS epialleles generated by trans-acting siRNAs did not change their active histone modifications.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Epigenetics
ISSN
1559-2294
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
666-676
UT code for WoS article
000327623100011
EID of the result in the Scopus database
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