Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle-dependent formation of gammaH2AX- and NBS1-positive repair foci
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00456060" target="_blank" >RIV/68081707:_____/15:00456060 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/boc.201500050" target="_blank" >http://dx.doi.org/10.1111/boc.201500050</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/boc.201500050" target="_blank" >10.1111/boc.201500050</a>
Alternative languages
Result language
angličtina
Original language name
Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle-dependent formation of gammaH2AX- and NBS1-positive repair foci
Original language description
BACKGROUND INFORMATION: The DNA damage response is a fundamental, well-regulated process that occurs in the genome to recognise DNA lesions. Here, we studied kinetics of proteins involved in DNA repair pathways and their recruitment to DNA lesions duringthe cell cycle. In non-irradiated and irradiated cells, we analysed the distribution pattern and spatiotemporal dynamics of gammaH2AX, 53BP1, BMI1, MDC1, NBS1, PCNA, coilin and BRCA1 proteins. RESULTS: We observed that spontaneous and irradiation-induced foci (IRIF) demonstrated a high abundance of phosphorylated H2AX, which was consistent with 53BP1 and BMI1 protein accumulation. However, NBS1 and MDC1 proteins were recruited to nuclear bodies (NBs) to a lesser extent. Irradiation by gamma-rays significantly increased the number of 53BP1- and gammaH2AX-positive IRIF, but cell cycle-dependent differences were only observed for gammaH2AX-positive foci in both non-irradiated and gamma-irradiated cells.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology of the Cell
ISSN
0248-4900
e-ISSN
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Volume of the periodical
107
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
440-454
UT code for WoS article
000368441100002
EID of the result in the Scopus database
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