Label-free electrochemical analysis of chitosan and glucosamine-containing oligosaccharides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00455881" target="_blank" >RIV/68081707:_____/16:00455881 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.electacta.2015.11.072" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.11.072</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2015.11.072" target="_blank" >10.1016/j.electacta.2015.11.072</a>

Result language

angličtina

Original language name

Label-free electrochemical analysis of chitosan and glucosamine-containing oligosaccharides

Original language description

Recently we have shown that, in difference to most of polysaccharides, which are considered as electroinactive, chitosan samples containing both glucosamine (GlcN) and N-acetylated glucosamine (GlcNAc) residues are involved in the catalytic hydrogen evolution reaction (CHER) at mercury electrodes, and can be determined by voltammetric or chronopotentiometric stripping. Here we show that polysaccharides and oligosaccharides composed of GlcN, but not those containing only GlcNAc residues, take part in CHER, allowing their easy label-free electrochemical detection. GlcNAc-containing oligosaccharides can be easily transformed into electroactive species by deacetylation using hot KOH treatment. This procedure can be applied also to electroinactive GlcNAc-containing glycans, frequently occurring in glycoproteins, allowing their electrochemical determination at submicromolar level. (C) 2015 Elsevier Ltd. All rights reserved.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA15-15479S" target="_blank" >GA15-15479S: New tools for research and diagnostics of diseases. Microfluidic reactors and electrochemistry for analysis of proteins and their glycosylation.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrochimica acta

ISSN

0013-4686

e-ISSN

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Volume of the periodical

187

Issue of the periodical within the volume

JAN 1 2016

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

375-380

UT code for WoS article

000367235600044

EID of the result in the Scopus database

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