HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00462034" target="_blank" >RIV/68081707:_____/16:00462034 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/16:00088134 RIV/00159816:_____/16:00065413

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0158358" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0158358</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0158358" target="_blank" >10.1371/journal.pone.0158358</a>

Result language

angličtina

Original language name

HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells

Original language description

Cardiac cell formation, cardiomyogenesis, is critically dependent on oxygen availability. It is known that hypoxia, a reduced oxygen level, modulates the in vitro differentiation of pluripotent cells into cardiomyocytes via hypoxia inducible factor-1alpha (HIF-1 alpha)-dependent mechanisms. However, the direct impact of HIF-1 alpha deficiency on the formation and maturation of cardiac-like cells derived from mouse embryonic stem cells (mESC) in vitro remains to be elucidated. In the present study, we demonstrated that HIF-1 alpha deficiency significantly altered the quality and quantity of mESC-derived cardiomyocytes. It was accompanied with lower mRNA and protein levels of cardiac cell specific markers (myosin heavy chains 6 and 7) and with a decreasing percentage of myosin heavy chain alpha and beta, and cardiac troponin T-positive cells. As to structural aspects of the differentiated cardiomyocytes, the localization of contractile proteins (cardiac troponin T, myosin heavy chain alpha and beta) and the organization of myofibrils were also different. Simultaneously, HIF-1 alpha deficiency was associated with a lower percentage of beating embryoid bodies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

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UT code for WoS article

000378859400053

EID of the result in the Scopus database

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