Electrochemical sensing of concanavalin A and ovalbumin interaction in solution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00463782" target="_blank" >RIV/68081707:_____/16:00463782 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.aca.2016.06.055" target="_blank" >http://dx.doi.org/10.1016/j.aca.2016.06.055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2016.06.055" target="_blank" >10.1016/j.aca.2016.06.055</a>

Result language

angličtina

Original language name

Electrochemical sensing of concanavalin A and ovalbumin interaction in solution

Original language description

In an attempt to develop a label- and reagent-free electrochemical method for the detection of lectin-glycoprotein interactions, we tested lectin-concanavalin A (ConA), glycoprotein-ovalbumin (Ova) and their complex using chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode. Incubation of ConA with Ova resulted in an increase of the CPS peak H of the complex as compared to the CPS peaks of individual Ova and ConA proteins. Qualitatively similar results were obtained with other glycoprotein-lectin couples (ConA-RNase B and lectin from Sambucus nigra-fetuin). Using the CPS method, we were able to follow the course of complex formation in solution. Comparable responses of Ova, ConA and ConA-Ova complex were obtained not only at the mercury electrode but also with solid amalgam electrodes, which are more suitable for parallel analysis. It can be anticipated that electrochemical methods, namely CPS, will find application in glycomics and proteomics. (C) 2016 Elsevier B.V. All rights reserved.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA13-00956S" target="_blank" >GA13-00956S: Emerging roles for the proteins of Anterior Gradient family in cancer development</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

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Volume of the periodical

935

Issue of the periodical within the volume

SEP2016

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

97-103

UT code for WoS article

000382249600007

EID of the result in the Scopus database

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