Hydrogen Evolution Facilitates Reduction of DNA Guanine Residues at the Hanging Mercury Drop Electrode: Evidence for a Chemical Mechanism

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00471945" target="_blank" >RIV/68081707:_____/16:00471945 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/16:00093645

Result on the web

<a href="http://dx.doi.org/10.1002/elan.201600242" target="_blank" >http://dx.doi.org/10.1002/elan.201600242</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201600242" target="_blank" >10.1002/elan.201600242</a>

Result language

angličtina

Original language name

Hydrogen Evolution Facilitates Reduction of DNA Guanine Residues at the Hanging Mercury Drop Electrode: Evidence for a Chemical Mechanism

Original language description

Guanine (G), as well as G residues in nucleosides, nucleotides and nucleic acids, undergo chemically reversible (but electrochemically irreversible) reduction/oxidation processes at the mercury-based electrodes. It has been established that G is reduced to 7,8-dihydroguanine at highly negative potentials. The reduction product is oxidized back to G around0.25V, giving rise to anodic peak G. Previous studies suggested involvement of a chemical mechanism involving electrochemically generated hydrogen radicals in the G reduction process. In this work we studied effects of cisplatin and pH on the G reduction process. We have found that catalytic hydrogen evolution accompanying electrochemical reduction of cisplatin markedly facilitates reduction of G. Minimum negative potential required for G reduction were shifted to less negative values and correlated with the onset of catalytic currents of cisplatin. Analogous shifts of the potential of G reduction were observed upon lowering pH of the background electrolyte (i.e., increasing the availability of protons to generate hydrogen radicals). Ammonium ions markedly increased efficiency of G reduction, which may be explained by generation of active hydrogen via formation and subsequent decomposition of ammonium amalgam. Our results strongly suggest that chemical mechanism(s) involving hydrogen radicals, electrochemically and/or electrocatalytically generated at the HMDE, contribute to the guanine 7,8-dihydroguanine conversion.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

2785-2790

UT code for WoS article

000387891400020

EID of the result in the Scopus database

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