Reproduction of the FC/DFC units in nucleoli

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00472029" target="_blank" >RIV/68081707:_____/16:00472029 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10327508

Result on the web

<a href="http://dx.doi.org/10.1080/19491034.2016.1157674" target="_blank" >http://dx.doi.org/10.1080/19491034.2016.1157674</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/19491034.2016.1157674" target="_blank" >10.1080/19491034.2016.1157674</a>

Result language

angličtina

Original language name

Reproduction of the FC/DFC units in nucleoli

Original language description

The essential structural components of the nucleoli, Fibrillar Centers (FC) and Dense Fibrillar Components (DFC), together compose FC/DFC units, loci of rDNA transcription and early RNA processing. In the present study we followed cell cycle related changes of these units in 2 human sarcoma derived cell lines with stable expression of RFP-PCNA (the sliding clamp protein) and GFP-RPA43 (a subunit of RNA polymerase I, pol I) or GFP-fibrillarin. Correlative light and electron microscopy analysis showed that the pol I and fibrillarin positive nucleolar beads correspond to individual FC/DFC units. In vivo observations showed that at early S phase, when transcriptionally active ribosomal genes were replicated, the number of the units in each cell increased by 60-80%. During that period the units transiently lost pol I, but not fibrillarin. Then, until the end of interphase, number of the units did not change, and their duplication was completed only after the cell division, by mid G1 phase. This peculiar mode of reproduction suggests that a considerable subset of ribosomal genes remain transcriptionally silent from mid S phase to mitosis, but become again active in the postmitotic daughter cells.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleus

ISSN

1949-1034

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

203-215

UT code for WoS article

000380238600011

EID of the result in the Scopus database

—