Butyrate alters expression of cytochrome P450 1A1 and metabolism of benzo[a] pyrene via its histone deacetylase activity in colon epithelial cell models
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00476270" target="_blank" >RIV/68081707:_____/17:00476270 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/17:00476270 RIV/00216224:14310/17:00097700 RIV/00027162:_____/17:N0000051
Result on the web
<a href="http://dx.doi.org/10.1007/s00204-016-1887-4" target="_blank" >http://dx.doi.org/10.1007/s00204-016-1887-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00204-016-1887-4" target="_blank" >10.1007/s00204-016-1887-4</a>
Alternative languages
Result language
angličtina
Original language name
Butyrate alters expression of cytochrome P450 1A1 and metabolism of benzo[a] pyrene via its histone deacetylase activity in colon epithelial cell models
Original language description
Butyrate, a short-chain fatty acid produced by fermentation of dietary fiber, is an important regulator of colonic epithelium homeostasis. In this study, we investigated the impact of this histone deacetylase (HDAC) inhibitor on expression/activity of cytochrome P450 family 1 (CYP1) and on metabolism of carcinogenic polycyclic aromatic hydrocarbon, benzo[a] pyrene (BaP), in colon epithelial cells. Sodium butyrate (NaBt) strongly potentiated the BaP-induced expression of CYP1A1 in human colon carcinoma HCT116 cells. It also co-stimulated the 7-ethoxyresorufin-O-deethylase (EROD) activity induced by the 2,3,7,8-tetrachlorodibenzo-p-dioxin, a prototypical ligand of the aryl hydrocarbon receptor. Up-regulation of CYP1A1 expression/activity corresponded with an enhanced metabolism of BaP and formation of covalent DNA adducts. NaBt significantly potentiated CYP1A1 induction and/or metabolic activation of BaP also in other human colon cell models, colon adenoma AA/C1 cells, colon carcinoma HT-29 cells, or in NCM460D cell line derived from normal colon mucosa. Our results suggest that the effects of NaBt were due to its impact on histone acetylation, because additional HDAC inhibitors (trichostatin A and suberanilohydroxamic acid) likewise increased both the induction of EROD activity and formation of covalent DNA adducts. NaBt-induced acetylation of histone II3 (at Lys14) and histone II4 (at Lys16), two histone modifications modulated during activation of CYP1A1 transcription, and it reduced binding of HDAC1 to the enhancer region of CYP1A1 gene. This in vitro study suggests that butyrate, through modulation of histone acetylation, may potentiate induction of CYP1A1 expression, which might in turn alter the metabolism of BaP within colon epithelial cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Toxicology
ISSN
0340-5761
e-ISSN
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Volume of the periodical
91
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
2135-2150
UT code for WoS article
000399875300008
EID of the result in the Scopus database
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