Immunoassays of chemically modified polysaccharides, glycans in glycoproteins and ribose in nucleic acids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00476719" target="_blank" >RIV/68081707:_____/17:00476719 - isvavai.cz</a>
Alternative codes found
RIV/00209805:_____/17:00077885
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2016.12.013" target="_blank" >http://dx.doi.org/10.1016/j.aca.2016.12.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2016.12.013" target="_blank" >10.1016/j.aca.2016.12.013</a>
Alternative languages
Result language
angličtina
Original language name
Immunoassays of chemically modified polysaccharides, glycans in glycoproteins and ribose in nucleic acids
Original language description
Glycosylation of proteins plays an important role in health and diseases. At present new simple and inexpensive methods of glycoprotein analysis are sought. We developed a monoclonal antibody Manost 2.1 in mice after immunization with the adduct of mannan with Os(VI)temed complex (temed is N,N,N',N'-tetramethylethylenediamine). The specificity of this antibody to different biomolecules treated with Os(VI)temed was tested using dot blot immunoassay. Manost 2.1 showed specificity toward Os(VI) temed-modified polysaccharides, glycoproteins and ribonucleotide at the 3'-end in DNA. The antibody recognized neither the unmodified compounds nor the non-glycosylated proteins treated with Os(VI) temed. We also performed western blotting and Coomassie silver blue staining of mixtures of biomacromolecules treated with Os(VI)temed and identified specifically the modified glycoproteins. The immunochemical method using Manost 2.1 was compared with electrochemical analyses based on redox signals of the Os(VI)temed adducts, with similar results in terms of sensitivity. This new antibody-based approach opens the door for rapid and inexpensive analysis of glycans and glycoproteins in various scientific and medical fields, including cancer research and the future application of glycoprotein detection in clinical practice. (C) 2016 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
955
Issue of the periodical within the volume
FEB2017
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
108-115
UT code for WoS article
000393242700010
EID of the result in the Scopus database
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