An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00485511" target="_blank" >RIV/68081707:_____/17:00485511 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.stemcr.2017.08.022" target="_blank" >http://dx.doi.org/10.1016/j.stemcr.2017.08.022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.stemcr.2017.08.022" target="_blank" >10.1016/j.stemcr.2017.08.022</a>
Alternative languages
Result language
angličtina
Original language name
An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells
Original language description
Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse ESCs, we generated a library of over 200 endogenously tagged fluorescent fusion proteins and present several proof-of-concept applications of this library. We show the utility of this library to track proteins in living cells, screen for pluripotency-related factors, identify heterogeneously expressing proteins, measure the dynamics of endogenously labeled proteins, track proteins recruited to sites of DNA damage, pull down tagged fluorescent fusion proteins using anti-Cherry antibodies, and test for interaction partners. Thus, this library can be used in a variety of different directions, either exploiting the fluorescent tag for imaging-based techniques or utilizing the fluorescent fusion protein for biochemical pull-down assays, including immunoprecipitation, co-immunoprecipitation, chromatin immunoprecipitation, and more.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem Cell Reports
ISSN
2213-6711
e-ISSN
—
Volume of the periodical
9
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1304-1314
UT code for WoS article
000412660000024
EID of the result in the Scopus database
—