Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00485552" target="_blank" >RIV/68081707:_____/17:00485552 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/17:73584447

Result on the web

<a href="http://dx.doi.org/10.1038/s41467-017-00631-3" target="_blank" >http://dx.doi.org/10.1038/s41467-017-00631-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41467-017-00631-3" target="_blank" >10.1038/s41467-017-00631-3</a>

Result language

angličtina

Original language name

Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination

Original language description

The cyclooxygenase-2 is a pro-inflammatory and cancer marker, whose mRNA stability and translation is regulated by the CUG-binding protein 2 interacting with AU-rich sequences in the 3 ' untranslated region. Here, we present the solution NMR structure of CUG-binding protein 2 RRM3 in complex with 5 '-UUUAA-3 ' originating from the COX-2 3 '-UTR. We show that RRM3 uses the same binding surface and protein moieties to interact with AU- and UG-rich RNA motifs, binding with low and high affinity, respectively. Using NMR spectroscopy, isothermal titration calorimetry and molecular dynamics simulations, we demonstrate that distinct sub-states characterized by different aromatic side-chain conformations at the RNA-binding surface allow for high-or low-affinity binding with functional implications. This study highlights a mechanism for RNA discrimination possibly common to multiple RRMs as several prominent members display a similar rearrangement of aromatic residues upon binding their targets.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Communications

ISSN

2041-1723

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

SEP2017

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

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UT code for WoS article

000411416100014

EID of the result in the Scopus database

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