gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity preliminary methodological study and discussion

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00485555" target="_blank" >RIV/68081707:_____/17:00485555 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1140/epjd/e2017-80073-2" target="_blank" >http://dx.doi.org/10.1140/epjd/e2017-80073-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1140/epjd/e2017-80073-2" target="_blank" >10.1140/epjd/e2017-80073-2</a>

Result language

angličtina

Original language name

gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity preliminary methodological study and discussion

Original language description

In order to improve patients' post-treatment quality of life, a shift from surgery to non-surgical (chemo) radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of gamma H2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of gamma-rays. To better understand complex tumor behavior, three different cell type primocultures CD90(-), CD90(+), and a mixed culture of these cells were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV-HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA16-12454S" target="_blank" >GA16-12454S: Characterizing & modifying complex response of head & neck tumor cells to different radiations - a step forward to combined personalized radiotherapy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Physical Journal D

ISSN

1434-6079

e-ISSN

—

Volume of the periodical

71

Issue of the periodical within the volume

9

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

—

UT code for WoS article

000411083200015

EID of the result in the Scopus database

—