Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00485557" target="_blank" >RIV/68081707:_____/17:00485557 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.elecom.2017.07.013" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2017.07.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.elecom.2017.07.013" target="_blank" >10.1016/j.elecom.2017.07.013</a>

Result language
angličtina
Original language name
Label-free detection of canonical DNA bases, uracil and 5-methylcytosine in DNA oligonucleotides using linear sweep voltammetry at a pyrolytic graphite electrode
Original language description
An innovative approach to label-free voltammetric analysis of DNA at a pyrolytic graphite electrode (PGE) within a broad range of potentials (from2.0 to + 1.6 V) in an acetate buffer (pH 5) is presented. Using specifically designed DNA nonamers, we demonstrate not only anodic oxidation, but for the first time also cathodic reduction of nucleobases at the PGE. In addition, products of irreversible oxidation/reduction of the parent bases are shown to yield analytically useful, base-specific cathodic/anodic signals, making it possible to distinguish between the canonical bases (adenine, cytosine, guanine and thymine), uracil (U) and 5-methylcytosine (mC) in DNA. Furthermore, selective electrochemical switching off of the redox signals specific to certain nucleobases is presented as a way to resolve overlapping signals. Similarly, newly reported signals corresponding to electrochemically transformed bases can be switched on under specific conditions. This approach can be utilized for fast and facile simultaneous label-free analysis of bases in DNA, including mC and U, and to uncover overlapping signals. This significantly extends the possible applications of PGE in DNA research and (bio) sensor development.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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