Multiparameter cytometric analysis of complex cellular response

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00488409" target="_blank" >RIV/68081707:_____/18:00488409 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/18:00068678 RIV/00216224:14310/18:00102432
Result on the web
<a href="http://dx.doi.org/10.1002/cyto.a.23295" target="_blank" >http://dx.doi.org/10.1002/cyto.a.23295</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.23295" target="_blank" >10.1002/cyto.a.23295</a>

Result language
angličtina
Original language name
Multiparameter cytometric analysis of complex cellular response
Original language description
Complex analysis of cellular responses after experimental treatment is important for screening, mechanistic understanding of treatment effects, and the identification of sensitive and resistant cell phenotypes. Modern multicolor flow cytometry has demonstrated its power for such analyses. Here, we introduce a multiparametric protocol for complex analysis of cytokinetics by the simultaneous detection of seven fluorescence parameters. This analysis includes the detection of two surface markers for immunophenotyping, analysis of proliferation based on the cell cycle and the measurement of incorporated nucleoside analogue 5-ethynyl-2-deoxyuridine (EdU) in newly synthesized DNA, analysis of DNA damage using an anti-phospho-histone H2A.X (Ser139) antibody, and determination of cell death using a fixable viability probe and intracellular detection of caspase-3 activation. To demonstrate the applicability of this protocol for the analysis of heterogeneous and complex cell responses, we used different treatments and model cell lines. We demonstrated that this protocol has the potential to provide complex and simultaneous analysis of cytokinetics and analyze the heterogeneity of the response at the single-cell level. (c) 2017 International Society for Advancement of Cytometry
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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