The fate of ribosomal RNA genes in spontaneous polyploid dogrose hybrids [Rosa L. sect. Caninae (DC.) Ser.] exhibiting non-symmetrical meiosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00492381" target="_blank" >RIV/68081707:_____/18:00492381 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/tpj.13843" target="_blank" >http://dx.doi.org/10.1111/tpj.13843</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.13843" target="_blank" >10.1111/tpj.13843</a>
Alternative languages
Result language
angličtina
Original language name
The fate of ribosomal RNA genes in spontaneous polyploid dogrose hybrids [Rosa L. sect. Caninae (DC.) Ser.] exhibiting non-symmetrical meiosis
Original language description
Dogroses represent an exceptional system for studying the effects of genome doubling and hybridization: their asymmetrical meiosis enables recombination in bi-parentally inherited chromosomes but prevents it in maternally inherited ones. We employed fluorescent insitu hybridization, genome skimming, amplicon sequencing of genomic and cDNA as well as conventional cloning of nuclear ribosomal DNA in two phylogenetically distinct pentaploid (2n=5x=35) species, Rosa canina and Rosainodora, and their naturally occurring reciprocal hybrids, Rosa dumalis (5x) and Rosa agrestis (5x, 6x). Both progenitor species differed in composition, meiotic behaviour and expression of rDNA loci: R.canina (five 18S and 5-8 5S loci) was dominated by the Canina ribotypes, but R.inodora (four 18S loci and 7-8 5S loci) by the Rubiginosa ribotype. The co-localized 5S/18S loci occurred on either bivalent-forming (R.canina) or univalent-forming (R.inodora) chromosomes. Ribosomal DNA loci were additively inherited, however, the Canina ribotypes were dominantly expressed, even in genotypes with relatively low copy number of these genes. Moreover, we observed rDNA homogenization towards the paternally transmitted Canina ribotype in 6x R.agrestis. The here-observed variation in arrangement and composition of rDNA types between R.canina and R.inodora suggests the involvement of different genomes in bivalent formation. This results supports the hypothesis that the asymmetrical meiosis arose at least twice by independent ancient hybridization events.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GC16-02149J" target="_blank" >GC16-02149J: Polyploidy generates beauty – exploration of complex genomes of roses (Rosa L. sect. Caninae (DC.) Ser.)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
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Volume of the periodical
94
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
77-90
UT code for WoS article
000428467900008
EID of the result in the Scopus database
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