Catalytic Deuterium Evolution and H/D Exchange in DNA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00504522" target="_blank" >RIV/68081707:_____/19:00504522 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/celc.201801214" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/celc.201801214</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/celc.201801214" target="_blank" >10.1002/celc.201801214</a>

Result language
angličtina
Original language name
Catalytic Deuterium Evolution and H/D Exchange in DNA
Original language description
Recently, it has been shown that DNA catalyzes the hydrogen evolution reaction (HER) at mercury-containing electrodes, which provide voltammetric and chronopotentiometric stripping (CPS) peaks H-DNA, allowing label-free DNA determination at very low concentrations. Here, we compared DNA catalytic HER in H2O with catalytic deuterium evolution reaction (DER) in D2O. We show that in D2O single-stranded (ss) and double-stranded (ds) DNAs produced CPS peaks H-DNA(D) due to DER. Compared to peaks H-DNA obtained in H2O, peaks H-DNA(D) were smaller and appeared at more negative potentials. Under conditions where dsDNA did not produce any peak H-DNA(D) in D2O, this DNA produced in H2O peak H-DNA.These results suggested a lack of accessible bases in the D2O surface-attached dsDNA. This might be attributed to the greater stability of deuterated dsDNA, better resisting the electric field effects at the negatively charged interface. The process of protonation of deuterated ssDNA was very fast in contrast to that of dsDNA, which was much slower and could be followed by CPS in a time range of tens of minutes.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/GA17-08971S" target="_blank" >GA17-08971S: Chemical modification and electrochemical analysis of nucleic acids as a new tool in molecular oncology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)