Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00518368" target="_blank" >RIV/68081707:_____/19:00518368 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/19:PU134306

Result on the web

<a href="http://dspace.lib.vutbr.cz/xmlui/bitstream/11012/184643/1/2019%20Electroanalsis%20Havranova%20Preprint.pdf" target="_blank" >http://dspace.lib.vutbr.cz/xmlui/bitstream/11012/184643/1/2019%20Electroanalsis%20Havranova%20Preprint.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201900306" target="_blank" >10.1002/elan.201900306</a>

Result language

angličtina

Original language name

Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex

Original language description

Alternative electrode materials suitable to prepare novel working electrode applicable in detecting biopolymers such as nucleic acids, proteins or glycoproteins, represent a significant contribution to bio-electroanalysis. Herein, electrodes made of vapor-deposited thin gold films (vAuE) were used as an alternative substrate for the electrodeposition of silver amalgam particles (AgAPs), next to indium tin oxide and pyrolytic graphite, which are already used. The conditions and parameters of double pulse chronoamperometry were optimized for the most-sensitive voltammetric detection of 4-nitrophenol (4-NP). The resulting electrodes were characterized by scanning electron microscope with energy dispersive X-ray spectroscopy. While 4-NP could not be detected by bare nonactivated vAuEs at all, their electrochemical activation offered a limit of detection (LoD) of 25 and 5 mu mol.l(-1) by means of CV and DPV, respectively. AgAP electrodeposited on vAuE, offered 2.5-times lower LoDs 10 mu mol.l(-1) by CV and comparable LoD 5 mu mol.l(-1) by DPV. Advantageously, AgAPs could be repeatedly deposited on and anodically dissolved from the vAuE with a relative standard deviation 13 % of the ten-times repeated DPV signal of 4-NP (100 mu mol.l(-1)). In comparison to vAuE, the vAuE-AgAP offered about 400 mV broader potential window, which allowed detection of single strand DNA fragment labeled by osmium tetroxide-bipyridine complex down to 2 ng.mu l(-1) by means of DPV.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

31

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1952-1960

UT code for WoS article

000474183900001

EID of the result in the Scopus database

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