HMGB2 is a negative regulator of telomerase activity in human embryonic stem and progenitor cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00522120" target="_blank" >RIV/68081707:_____/19:00522120 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/19:00108537 RIV/00159816:_____/19:00072485
Result on the web
<a href="https://www.fasebj.org/doi/pdf/10.1096/fj.201901465RRR" target="_blank" >https://www.fasebj.org/doi/pdf/10.1096/fj.201901465RRR</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1096/fj.201901465RRR" target="_blank" >10.1096/fj.201901465RRR</a>
Alternative languages
Result language
angličtina
Original language name
HMGB2 is a negative regulator of telomerase activity in human embryonic stem and progenitor cells
Original language description
High-mobility group box (HMGB)1 and HMGB2 proteins are the subject of intensive research because of their involvement in DNA replication, repair, transcription, differentiation, proliferation, cell signaling, inflammation, and tumor migration. Using inducible, stably transfected human embryonic stem cells (hESCs) capable of the short hairpin RNA-mediated knockdown (KD) of HMGB1 and HMGB2, we provide evidence that deregulation of HMGB1 or HMGB2 expression in hESCs and their differentiated derivatives (neuroectodermal cells) results in distinct modulation of telomere homeostasis. Whereas HMGB1 enhances telomerase activity, HMGB2 acts as a negative regulator of telomerase activity in the cell. Stimulation of telomerase activity in the HMGB2-deficient cells may be related to activation of the PI3K/protein kinase B/ glycogen synthase kinase-313/ I3-catenin signaling pathways by HMGB1, augmented TERT/telomerase RNA subunit transcription, and possibly also because of changes in telomeric repeat-containing RNA (TERRA) and TERRA-polyA(+) transcription. The impact of HMGB1/2 KD on telomerase transcriptional regulation observed in neuroectodermal cells is partially masked in hESCs by their pluripotent state. Our findings on differential roles of HMGB1 and HMGB2 proteins in regulation of telomerase activity may suggest another possible outcome of HMGB1 targeting in cells, which is currently a promising approach aiming at increasing the anticancer activity of cytotoxic agents.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FASEB Journal
ISSN
0892-6638
e-ISSN
1530-6860
Volume of the periodical
33
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
14307-14324
UT code for WoS article
000507466100097
EID of the result in the Scopus database
2-s2.0-85076061677