Stabilization of human telomeric RNA G-quadruplex by the water-compatible optically pure and biologically-active metallohelices

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00536122" target="_blank" >RIV/68081707:_____/20:00536122 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-020-71429-5.pdf" target="_blank" >https://www.nature.com/articles/s41598-020-71429-5.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-020-71429-5" target="_blank" >10.1038/s41598-020-71429-5</a>

Result language
angličtina
Original language name
Stabilization of human telomeric RNA G-quadruplex by the water-compatible optically pure and biologically-active metallohelices
Original language description
RNA G-quadruplexes have been suggested to play key roles in fundamental biological processes and are linked to human diseases. Thus, they also represent good potential therapeutic targets. Here, we describe, using the methods of molecular biophysics, interactions of a series of biologically-active supramolecular cationic metallohelices with human telomeric RNA G-quadruplex. We demonstrate that the investigated metallohelices bind with a high affinity to human telomeric RNA G-quadruplex and that their binding selectivity considerably differs depending on the dimensions and overall shape of the metallohelices. Additionally, the investigated metallohelices inhibit DNA synthesis on the RNA template containing four repeats of the human telomeric sequence by stabilizing the RNA G-quadruplex structure. Collectively, the results of this study suggest that stabilization of RNA sequences capable of G-quadruplex formation by metallohelices investigated in this work might contribute to the mechanism of their biological activity.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10700 - Other natural sciences

Project
<a href="/en/project/GA20-00735S" target="_blank" >GA20-00735S: Metallo-supramolecular helicates as DNA condensing agents and carriers for DNA delivery</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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