Human Papillomavirus G-Rich Regions as Potential Antiviral Drug Targets

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00536202" target="_blank" >RIV/68081707:_____/20:00536202 - isvavai.cz</a>
Result on the web
<a href="https://www.liebertpub.com/doi/full/10.1089/nat.2020.0869" target="_blank" >https://www.liebertpub.com/doi/full/10.1089/nat.2020.0869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/nat.2020.0869" target="_blank" >10.1089/nat.2020.0869</a>

Result language
angličtina
Original language name
Human Papillomavirus G-Rich Regions as Potential Antiviral Drug Targets
Original language description
Herein, we report, for the first time, the screening of several ligands in terms of their ability to bind and stabilize G-quadruplexes (G4) found in seven human Papillomavirus (HPV) genomes. Using a variety of biophysical assays, HPV G-quadruplexes were shown to possess a high degree of structural polymorphism upon ligand binding, which may have an impact on transcription, replication, and viral protein production. A sequence found in high-risk HPV16 genotype folds into multiple non-canonical DNA structures, it was converted into a major G4 conformation upon interaction with a well-characterized highly selective G4 ligand, PhenDC3, which may have an impact on the viral infection. Likewise, HPV57 and 58, which fold into multiple G4 structures, were found to form single stable complexes in the presence of two other G4 ligands, C-8 and pyridostatin, respectively. In addition, one of the selected compounds, the acridine derivative C-8, demonstrated a significant antiviral effect in HPV18-infected organotypic raft cultures. Altogether, these results indicate that targeting HPV G4s may be an alternative route for the development of novel antiviral therapies.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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