The fate of 35S rRNA genes in the allotetraploid grassBrachypodium hybridum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00539338" target="_blank" >RIV/68081707:_____/20:00539338 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14869" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.14869" target="_blank" >10.1111/tpj.14869</a>
Alternative languages
Result language
angličtina
Original language name
The fate of 35S rRNA genes in the allotetraploid grassBrachypodium hybridum
Original language description
Nucleolar dominance (ND) consists of the reversible silencing of 35S/45S rDNA loci inherited from one of the ancestors of an allopolyploid. The molecular mechanisms by which one ancestral rDNA set is selected for silencing remain unclear. We applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequence analysis), genomic (analysis of variants) and cytogenetic (fluorescencein situhybridization) approaches to study the structure, expression and epigenetic landscape of 35S rDNA in an allotetraploid grass that exhibits ND,Brachypodium hybridum(genome composition DDSS), and its putative progenitors,Brachypodium distachyon(DD) andBrachypodium stacei(SS). In progenitor genomes,B. staceishowed a higher intragenomic heterogeneity of rDNA compared withB. distachyon. In all studied accessions ofB. hybridum, there was a reduction in the copy number of S homoeologues, which was accompanied by their inactive transcriptional status. The involvement of DNA methylation in CG and CHG contexts in the silencing of the S-genome rDNA loci was revealed. In theB. hybridumallotetraploid, ND is stabilized towards the D-genome units, irrespective of the polyphyletic origin of the species, and does not seem to be influenced by homoeologous 35S rDNA ratios and developmental stage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GC20-14133J" target="_blank" >GC20-14133J: When sex may or may not happen – The impact of asymmetrical meiosis on the evolutionary fate of genes in polyploid roses</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
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Volume of the periodical
103
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
1810-1825
UT code for WoS article
000545082000001
EID of the result in the Scopus database
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