Peptide-Based Identification of Phytophthora Isolates and Phytophthora Detection in Planta
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00540057" target="_blank" >RIV/68081707:_____/20:00540057 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/21/24/9463" target="_blank" >https://www.mdpi.com/1422-0067/21/24/9463</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21249463" target="_blank" >10.3390/ijms21249463</a>
Alternative languages
Result language
angličtina
Original language name
Peptide-Based Identification of Phytophthora Isolates and Phytophthora Detection in Planta
Original language description
Phytophthora is arguably one of the most damaging genera of plant pathogens. This pathogen is well suited to transmission via the international plant trade, and globalization has been promoting its spread since the 19th century. Early detection is essential for reducing its economic and ecological impact. Here, a shotgun proteomics approach was utilized for Phytophthora analysis. The collection of 37 Phytophthora isolates representing 12 different species was screened for species-specific peptide patterns. Next, Phytophthora proteins were detected in planta, employing model plants Solanum tuberosum and Hordeum vulgare. Although the evolutionarily conserved sequences represented more than 10% of the host proteome and limited the pathogen detection, the comparison between qPCR and protein data highlighted more than 300 protein markers, which correlated positively with the amount of P. infestans DNA. Finally, the analysis of P. palmivora response in barley revealed significant alterations in plant metabolism. These changes included enzymes of cell wall metabolism, ROS production, and proteins involved in trafficking. The observed root-specific attenuation in stress-response mechanisms, including the biosynthesis of jasmonates, ethylene and polyamines, and an accumulation of serotonin, provided the first insight into molecular mechanisms behind this particular biotic interaction.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
24
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
9463
UT code for WoS article
000602791500001
EID of the result in the Scopus database
2-s2.0-85097657663