In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00536199" target="_blank" >RIV/68081707:_____/21:00536199 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/21:00118818
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/anie.202007184" target="_blank" >https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/anie.202007184</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.202007184" target="_blank" >10.1002/anie.202007184</a>
Alternative languages
Result language
angličtina
Original language name
In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells
Original language description
We report here the in-cell NMR-spectroscopic observation of the binding of the cognate ligand 2 '-deoxyguanosine to the aptamer domain of the bacterial 2 '-deoxyguanosine-sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes and in human HeLa cells. The riboswitch is sufficiently stable in both cell types to allow for detection of binding of the ligand to the riboswitch. Most importantly, we show that the binding mode established by in vitro characterization of this prokaryotic riboswitch is maintained in eukaryotic cellular environment. Our data also bring important methodological insights: Thus far, in-cell NMR studies on RNA in mammalian cells have been limited to investigations of short (<15 nt) RNA fragments that were extensively modified by protecting groups to limit their degradation in the intracellular space. Here, we show that the in-cell NMR setup can be adjusted for characterization of much larger (approximate to 70 nt) functional and chemically non-modified RNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Angewandte Chemie - International Edition
ISSN
1433-7851
e-ISSN
1521-3773
Volume of the periodical
60
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
865-872
UT code for WoS article
000591274200001
EID of the result in the Scopus database
2-s2.0-85096655060