WALTER: an easy way to online evaluate telomere lengths from terminal restriction fragment analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00542027" target="_blank" >RIV/68081707:_____/21:00542027 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/21:00119108
Result on the web
<a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04064-0" target="_blank" >https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04064-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12859-021-04064-0" target="_blank" >10.1186/s12859-021-04064-0</a>
Alternative languages
Result language
angličtina
Original language name
WALTER: an easy way to online evaluate telomere lengths from terminal restriction fragment analysis
Original language description
BackgroundTelomeres, nucleoprotein structures comprising short tandem repeats and delimiting the ends of linear eukaryotic chromosomes, play an important role in the maintenance of genome stability. Therefore, the determination of the length of telomeres is of high importance for many studies. Over the last years, new methods for the analysis of the length of telomeres have been developed, including those based on PCR or analysis of NGS data. Despite that, terminal restriction fragment (TRF) method remains the gold standard to this day. However, this method lacks universally accepted and precise tool capable to analyse and statistically evaluate TRF results.ResultsTo standardize the processing of TRF results, we have developed WALTER, an online toolset allowing rapid, reproducible, and user-friendly analysis including statistical evaluation of the data. Given its web-based nature, it provides an easily accessible way to analyse TRF data without any need to install additional software.ConclusionsWALTER represents a major upgrade from currently available tools for the image processing of TRF scans. This toolset enables a rapid, highly reproducible, and user-friendly evaluation of almost any TRF scan including in-house statistical evaluation of the data. WALTER platform together with user manual describing the evaluation of TRF scans in detail and presenting tips and troubleshooting, as well as test data to demo the software are available at https://www.ceitec.eu/chromatin-molecular-complexes-jiri-fajkus/rg51/tab?tabId=125#WALTER and the source code at https://github.com/mlyc93/WALTER.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Bioinformatics
ISSN
1471-2105
e-ISSN
1471-2105
Volume of the periodical
22
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
14
Pages from-to
145
UT code for WoS article
000634776700003
EID of the result in the Scopus database
2-s2.0-85102696153