Evolution of plant telomerase RNAs: farther to the past, deeper to the roots

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00554846" target="_blank" >RIV/68081707:_____/21:00554846 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/21:00554846 RIV/00216224:14740/21:00119679

Result on the web

<a href="https://academic.oup.com/nar/article/49/13/7680/6310787" target="_blank" >https://academic.oup.com/nar/article/49/13/7680/6310787</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkab545" target="_blank" >10.1093/nar/gkab545</a>

Result language

angličtina

Original language name

Evolution of plant telomerase RNAs: farther to the past, deeper to the roots

Original language description

The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs. linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomere repeats.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

1362-4962

Volume of the periodical

49

Issue of the periodical within the volume

13

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

7680-7694

UT code for WoS article

000685211300038

EID of the result in the Scopus database

2-s2.0-85112126900