Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F22%3A00562612" target="_blank" >RIV/68081707:_____/22:00562612 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/22:00128593

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2022.111453" target="_blank" >10.1016/j.plantsci.2022.111453</a>

Result language

angličtina

Original language name

Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses

Original language description

ARM was identified previously as an interaction partner of the telomerase protein subunit (TERT) in Arabidopsis thaliana. To investigate the interconnection between ARM and telomerase and to identify ARM cellular functions, we analyzed a set of arm mutant lines and arm/tert double mutants. Telomere length was not affected in arm single mutant plants, in contrast to double mutants. In the second generation of homozygous arm-1/tert double mutants following the heterozygous state during the double mutant construction, telomeres shortened dramat-ically, even below levels in tert plants displaying severe morphological defects. Intriguingly, homozygous arm-1/ tert double mutants with short telomeres grew without obvious phenotypic changes for next two generations. Then, in agreement with the onset of phenotypic changes in tert, morphological defects were timed to the 5th arm-1/tert homozygous generation. RNAseq analyses of arm-1/tert and respective single mutants displayed markedly overlapping sets of differentially expressed genes in arm-1/tert double mutant and arm-1 single mutant lines, indicating a dominant effect of the ARM mutation. RNAseq data further implied ARM involvement in circadian rhythms, responses to drugs and to biotic and abiotic stimuli. In agreement with it, we observed sensitivity of arm-1 single mutant to the heat stress during germination. Altogether, our results suggest ARM involvement in crucial cellular processes without evidencing its role in the telomerase canonical function.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

1873-2259

Volume of the periodical

325

Issue of the periodical within the volume

DEC 2022

Country of publishing house

IE - IRELAND

Number of pages

16

Pages from-to

111453

UT code for WoS article

000862382300002

EID of the result in the Scopus database

2-s2.0-85138552413