Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00568949" target="_blank" >RIV/68081707:_____/23:00568949 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00130873
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0013468623000658?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0013468623000658?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2023.141878" target="_blank" >10.1016/j.electacta.2023.141878</a>
Alternative languages
Result language
angličtina
Original language name
Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes
Original language description
Development of analytical tools enabling discrimination amongst various structures of nucleic acids attracts interest due to diverse biological functions and prospective biotechnological applications of the latter. In this work, two electrochemically active compounds, copper porphyrin complex Cu-TMPy2PP and base-modified deoxyguanosine triphosphate conjugate bearing 3-nitrophenyl moiety (dGNPTP), were used as probes to study electrode potential effects on oligodeoxynucleotides (ODNs) of various sequences and abilities to form secondary structures (including unstructured single strands, duplexes, guanine quadruplexes, i-motifs) at the surface of hanging mercury drop electrode (HMDE). Our experiments revealed formation of less dense adsorbed layers by G-rich ODNs with the propensity to fold into G-quadruplexes (G4), compared to layers formed by other studied sequences. Strikingly, the G4-forming ODNs were significantly more susceptible to desorption from the HMDE surface during its polarization to highly negative potentials compared to other ODNs. Negative potential-induced desorption of the ODNs from HMDE was further confirmed by gel electrophoresis. Application of the electro-chemical probes offers a useful method for further research of DNA conformations and transitions taking place in solution and at electrically charged surfaces.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica acta
ISSN
0013-4686
e-ISSN
1873-3859
Volume of the periodical
442
Issue of the periodical within the volume
FEB 20 2023
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
141878
UT code for WoS article
000923609000001
EID of the result in the Scopus database
2-s2.0-85146151102