Structural and dynamic effects of pseudouridine modifications on noncanonical interactions in RNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00574517" target="_blank" >RIV/68081707:_____/23:00574517 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/23:00130997
Result on the web
<a href="https://rnajournal.cshlp.org/content/29/6/790" target="_blank" >https://rnajournal.cshlp.org/content/29/6/790</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.079506.122" target="_blank" >10.1261/rna.079506.122</a>
Alternative languages
Result language
angličtina
Original language name
Structural and dynamic effects of pseudouridine modifications on noncanonical interactions in RNA
Original language description
Pseudouridine is the most frequently naturally occurring RNA modification, found in all classes of biologically functional RNAs. Compared to uridine, pseudouridine contains an additional hydrogen bond donor group and is therefore widely regarded as a structure stabilizing modification. However, the effects of pseudouridine modifications on the structure and dynamics of RNAs have so far only been investigated in a limited number of different structural contexts. Here, we introduced pseudouridine modifications into the U-turn motif and the adjacent U:U closing base pair of the neomycin-sensing riboswitch (NSR)-an extensively characterized model system for RNA structure, ligand binding, and dynamics. We show that the effects of replacing specific uridines with pseudouridines on RNA dynamics crucially depend on the exact location of the replacement site and can range from destabilizing to locally or even globally stabilizing. By using a combination of NMR spectroscopy, MD simulations and QM calculations, we rationalize the observed effects on a structural and dynamical level. Our results will help to better understand and predict the consequences of pseudouridine modifications on the structure and function of biologically important RNAs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA23-05639S" target="_blank" >GA23-05639S: Molecular dynamics simulations of RNA: from static structures to molecular ensembles</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RNA
ISSN
1355-8382
e-ISSN
1469-9001
Volume of the periodical
29
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
790-807
UT code for WoS article
000989437200007
EID of the result in the Scopus database
2-s2.0-85159738407