The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast<i> Saccharomyces</i> cerevisiae
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00583206" target="_blank" >RIV/68081707:_____/23:00583206 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/23:PU149837
Result on the web
<a href="https://portlandpress.com/bioscirep/article/43/12/BSR20231348/233764/The-presence-of-a-G-quadruplex-prone-sequence" target="_blank" >https://portlandpress.com/bioscirep/article/43/12/BSR20231348/233764/The-presence-of-a-G-quadruplex-prone-sequence</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1042/BSR20231348" target="_blank" >10.1042/BSR20231348</a>
Alternative languages
Result language
angličtina
Original language name
The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast<i> Saccharomyces</i> cerevisiae
Original language description
Non-canonical secondary structures in DNA are increasingly being revealed as critical players in DNA metabolism, including modulating the accessibility and activity of promoters. These structures comprise the so-called G-quadruplexes (G4s) that are formed from sequences rich in guanine bases. Using a well-defined transcriptional reporter system, we sought to systematically investigate the impact of the presence of G4 structures on transcription in yeast Saccharomyces cerevisiae. To this aim, different G4 prone sequences were modeled to vary the chance of intramolecular G4 formation, analyzed in vitro by Thioflavin T binding test and circular dichroism and then placed at the yeast ADE2 locus on chromosome XV, downstream and adjacent to a P53 response element (RE) and upstream from a minimal CYC1 promoter and Luciferase 1 (LUC1) reporter gene in isogenic strains. While the minimal CYC1 promoter provides basal reporter activity, the P53 RE enables LUC1 transactivation under the control of P53 family proteins expressed under the inducible GAL1 promoter. Thus, the impact of the different G4 prone sequences on both basal and P53 family protein-dependent expression was measured after shifting cells onto galactose containing medium. The results showed that the presence of G4 prone sequences upstream of a yeast minimal promoter increased its basal activity proportionally to their potential to form intramolecular G4 structures, consequently, this feature, when present near the target binding site of P53 family transcription factors, can be exploited to regulate the transcriptional activity of P53, P63 and P73 proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA22-21903S" target="_blank" >GA22-21903S: Local DNA structures and their role in mutant p53 protein function in human tumours</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioscience Reports
ISSN
0144-8463
e-ISSN
1573-4935
Volume of the periodical
43
Issue of the periodical within the volume
12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
BSR20231348
UT code for WoS article
001128595000001
EID of the result in the Scopus database
2-s2.0-85180767783