RNA-related DNA damage and repair: The role of N7-methylguanosine in the cell nucleus exposed to UV light
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00584665" target="_blank" >RIV/68081707:_____/24:00584665 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S240584402401630X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S240584402401630X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.heliyon.2024.e25599" target="_blank" >10.1016/j.heliyon.2024.e25599</a>
Alternative languages
Result language
angličtina
Original language name
RNA-related DNA damage and repair: The role of N7-methylguanosine in the cell nucleus exposed to UV light
Original language description
Background: Chemical modifications in mRNAs, tRNAs, rRNAs, and noncoding RNAs stabilize these nucleic acids and regulate their function. In addition to regulating the translation of genetic information from mRNA to proteins, it has been revealed that modifications in RNAs regulate repair processes in the genome. Methods: Using local laser microirradiation, confocal microscopy, dot blots, and mass spectrometry we studied the role of N7-methylguanosine (m7G), which is cotranscriptionally installed in RNA. Results: Here, we show that after UVC and UVA irradiation, the level of m7G RNA is increased initially in the cytoplasm, and after local laser microirradiation, m7G RNA is highly abundant in UVA-damaged chromatin. This process is poly(ADP-ribose) polymerase (PARP)-dependent, but not accompanied by changes in the level of m7G-writers, including methyltransferases RNMT, METTL1, and WBSCR22. We also observed that METTL1 deficiency does not affect the recruitment of m7G RNA to microirradiated chromatin. Analyzing the levels of mRNA, let7e, and miR203a in both the cytoplasm and the cell nucleus, we revealed that UVC irradiation changed the level of mRNA, and significantly increased the pool of both let7e and miR-203a, which correlated with radiationinduced m7G RNA increase in the cytoplasm. Conclusions: Irradiation by UV light increases the m7G RNA pool in the cytoplasm and in the microirradiated genome. Thus, epigenetically modified RNAslikely contribute to DNA damage responses or m7G signals the presence of RNA damage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
<a href="/en/project/GM22-20303M" target="_blank" >GM22-20303M: Deciphering Origins of DNA replication in genome integrity</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Heliyon
ISSN
2405-8440
e-ISSN
2405-8440
Volume of the periodical
10
Issue of the periodical within the volume
4
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
15
Pages from-to
e25599
UT code for WoS article
001184241500001
EID of the result in the Scopus database
2-s2.0-85184755108